Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | genetic association 0.28 (overall 0.20) | 0.28 |
| cell type Human Protein Atlas | expressed broadly, no clear home in brain cells | 0.40 |
| chemistry Open Targets | a small-molecule pocket | 0.60 |
| trials ClinicalTrials.gov | no Parkinson's trials on record: an open field | 1.00 |
| literature PubMed | 32 Parkinson's papers of 1,311 | 0.54 |
| pathways Reactome | 13 pathways; mostly mitochondrial quality control | 1.00 |
Knock down and overexpress PPARGC1A in a dopamine neuron, astrocyte and microglia tri-culture; measure PINK1–parkin mitophagy flux (mt-Keima) and phospho-ubiquitin (pS65-Ub) and dopamine neuron survival after α-synuclein fibril challenge.
- Cell type
- a dopamine neuron, astrocyte and microglia tri-culture
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- PINK1–parkin mitophagy flux (mt-Keima) and phospho-ubiquitin (pS65-Ub)
- Functional readout
- dopamine neuron survival after α-synuclein fibril challenge
- Rule-out
- If neither arm moves PINK1–parkin mitophagy flux (mt-Keima) by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of PPARGC1A in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: PINK1–parkin mitophagy flux (mt-Keima) and phospho-ubiquitin (pS65-Ub); dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Sealed predictions
- PPARGC1A in dopamine neurons, braak+0.31 (p 0.414) → +0.85 (p 0.004)receipt 2017
Swarm cases
receipt 2017: PPARGC1A in dopamine neurons, braak
- power
- full sample +0.56 (p 0.015, n 18), 95% CI +0.13 to +0.82. the interval excludes zero: something may be there, smaller than a half can see.
- confounder
- adjusted for sex: +0.53 (p 0.025, n 18). holds up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: +0.53 (p 0.025, n 18). not the mix.
- skeptic
- leave-one-out range +0.52 to +0.71, sign flips 0; without severely affected donors +0.56 (p 0.015, n 18). robust.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for PPARGC1A in dopamine neurons.
case closed: nothing better to follow up.
Receipts
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