Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | no genetic evidence; overall 0.01 from other evidence | 0.00 |
| cell type Human Protein Atlas | expressed broadly, no clear home in brain cells | 0.40 |
| chemistry Open Targets | compounds already reach it: abequolixron, rovazolac, cs-8080 +3 | 0.90 |
| trials ClinicalTrials.gov | no Parkinson's trials on record: an open field | 1.00 |
| literature PubMed | 2 Parkinson's papers of 295 | 0.85 |
| pathways Reactome | 10 pathways; mostly protein handling | 0.90 |
Knock down and overexpress NR1H3 in a dopamine neuron, astrocyte and microglia tri-culture; measure proteasome activity and the load of ubiquitinated protein and dopamine neuron survival after α-synuclein fibril challenge.
- Cell type
- a dopamine neuron, astrocyte and microglia tri-culture
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- proteasome activity and the load of ubiquitinated protein
- Functional readout
- dopamine neuron survival after α-synuclein fibril challenge
- Rule-out
- If neither arm moves proteasome activity by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of NR1H3 in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: proteasome activity and the load of ubiquitinated protein; dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Sealed predictions
- NR1H3 in dopamine neurons, braak-0.84 (p 0.005) → +0.27 (p 0.485)receipt 9239
Swarm cases
receipt 9239: NR1H3 in dopamine neurons, braak
- power
- full sample -0.46 (p 0.057, n 18), 95% CI -0.76 to +0.01. the interval is wide: not enough donors to tell.
- confounder
- adjusted for sex: -0.48 (p 0.046, n 18). holds up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: -0.58 (p 0.011, n 18). not the mix.
- skeptic
- leave-one-out range -0.54 to -0.39, sign flips 0; without severely affected donors -0.46 (p 0.057, n 18). robust.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for NR1H3 in dopamine neurons.
case closed: nothing better to follow up.
Receipts
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