Braak stage 1, 100% of pigmented neurons left
parkinsonconnecting

Gene record

GALNT7

polypeptide N-acetylgalactosaminyltransferase 7

translation score 0.56, read 2026-10-18; 1 sealed predictions, 0 held; 1 receipts.

Six readers

readerfoundscore
genetics
Open Targets		genetic association 0.38 (overall 0.23)0.38
cell type
Human Protein Atlas		expressed broadly, no clear home in brain cells0.40
chemistry
Open Targets		a small-molecule pocket0.60
trials
ClinicalTrials.gov		no Parkinson's trials on record: an open field1.00
literature
PubMed		1 Parkinson's papers of 720.91
pathways
Reactome		1 pathway; mostly other0.30
proposal for GALNT7, translation score 0.56

Knock down and overexpress GALNT7 in a dopamine neuron, astrocyte and microglia tri-culture; measure a transcriptome-wide state shift (bulk RNA-seq) and dopamine neuron survival after α-synuclein fibril challenge.

Cell type
a dopamine neuron, astrocyte and microglia tri-culture
Direction
Both arms: public data does not say which way helps.
Molecular readout
a transcriptome-wide state shift (bulk RNA-seq)
Functional readout
dopamine neuron survival after α-synuclein fibril challenge
Rule-out
If neither arm moves a transcriptome-wide state shift (bulk RNA-seq) by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
Lab request
CRISPRi knockdown and lentiviral overexpression of GALNT7 in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: a transcriptome-wide state shift (bulk RNA-seq); dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.

Sealed predictions

  1. GALNT7 in dopamine neurons, braak+0.30 (p 0.426) → -0.04 (p 0.924)receipt 1868

Swarm cases

receipt 1868: GALNT7 in dopamine neurons, braak

power
full sample -0.02 (p 0.949, n 18), 95% CI -0.48 to +0.45. the interval is wide: not enough donors to tell.
confounder
adjusted for sex: -0.00 (p 0.997, n 18). does not hold up.
composition
dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: -0.01 (p 0.969, n 18). could be the mix.
skeptic
leave-one-out range -0.08 to +0.10, sign flips 6; without severely affected donors -0.02 (p 0.949, n 18). fragile.
cell type
no other cell group does better.
axis
no measure beats "tracks Braak Lewy-body stage" for GALNT7 in dopamine neurons.

case closed: nothing better to follow up.

Receipts

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