Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | genetic association 0.37 (overall 0.26) | 0.37 |
| cell type Human Protein Atlas | expressed broadly, no clear home in brain cells | 0.40 |
| chemistry Open Targets | reachable by antibody | 0.36 |
| trials ClinicalTrials.gov | no Parkinson's trials on record: an open field | 1.00 |
| literature PubMed | 32 Parkinson's papers of 836 | 0.54 |
| pathways Reactome | 1 pathway; mostly lysosome and autophagy | 1.00 |
Knock down and overexpress GALC in a dopamine neuron, astrocyte and microglia tri-culture; measure glucocerebrosidase (GCase) activity and LC3-II and p62 flux and dopamine neuron survival after α-synuclein fibril challenge.
- Cell type
- a dopamine neuron, astrocyte and microglia tri-culture
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- glucocerebrosidase (GCase) activity and LC3-II and p62 flux
- Functional readout
- dopamine neuron survival after α-synuclein fibril challenge
- Rule-out
- If neither arm moves glucocerebrosidase (GCase) activity by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of GALC in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: glucocerebrosidase (GCase) activity and LC3-II and p62 flux; dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Sealed predictions
- GALC in dopamine neurons, braak+0.64 (p 0.065) → +0.57 (p 0.108)receipt 1559
In the sealed studies
- The lysosomal axis in dopamine neurons: did not hold
Swarm cases
receipt 1559: GALC in dopamine neurons, braak
- power
- full sample +0.64 (p 0.0039, n 18), 95% CI +0.25 to +0.85. the interval excludes zero: something may be there, smaller than a half can see.
- confounder
- adjusted for sex: +0.66 (p 0.0030, n 18). holds up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: +0.68 (p 0.0017, n 18). not the mix.
- skeptic
- leave-one-out range +0.61 to +0.70, sign flips 0; without severely affected donors +0.64 (p 0.0039, n 18). robust.
- cell type
- no other cell group does better.
- axis
- no measure beats "tracks Braak Lewy-body stage" for GALC in dopamine neurons.
case closed: nothing better to follow up.
Receipts
loading the receipts