Braak stage 1, 100% of pigmented neurons left
parkinsonconnecting

Gene record

CERT1

ceramide transporter 1

translation score 0.56, read 2026-10-23; 1 sealed predictions, 0 held; 1 receipts.

Six readers

readerfoundscore
genetics
Open Targets		no genetic evidence; overall 0.02 from other evidence0.00
cell type
Human Protein Atlas		expressed broadly, no clear home in brain cells0.40
chemistry
Open Targets		a small-molecule pocket0.60
trials
ClinicalTrials.gov		no Parkinson's trials on record: an open field1.00
literature
PubMed		no papers tie it to Parkinson's yet1.00
pathways
Reactome		1 pathway; mostly lysosome and autophagy1.00
proposal for CERT1, translation score 0.56

Knock down and overexpress CERT1 in a dopamine neuron, astrocyte and microglia tri-culture; measure glucocerebrosidase (GCase) activity and LC3-II and p62 flux and dopamine neuron survival after α-synuclein fibril challenge.

Cell type
a dopamine neuron, astrocyte and microglia tri-culture
Direction
Both arms: public data does not say which way helps.
Molecular readout
glucocerebrosidase (GCase) activity and LC3-II and p62 flux
Functional readout
dopamine neuron survival after α-synuclein fibril challenge
Rule-out
If neither arm moves glucocerebrosidase (GCase) activity by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
Lab request
CRISPRi knockdown and lentiviral overexpression of CERT1 in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: glucocerebrosidase (GCase) activity and LC3-II and p62 flux; dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.

Sealed predictions

  1. CERT1 in dopamine neurons, braakn/a → n/areceipt 8036

Swarm cases

receipt 8036: CERT1 in dopamine neurons, braak

cell type
CERT1 is not measured in dopamine neurons; looking elsewhere.
cell type
no other cell group does better.
axis
no measure beats "tracks Braak Lewy-body stage" for CERT1 in dopamine neurons.

case closed: nothing better to follow up.

Receipts

loading the receipts