Six readers
| reader | found | score |
|---|---|---|
| genetics Open Targets | genetic association 0.84 (overall 0.54) | 0.84 |
| cell type Human Protein Atlas | expressed broadly, no clear home in brain cells | 0.40 |
| chemistry Open Targets | a small-molecule pocket | 0.60 |
| trials ClinicalTrials.gov | no Parkinson's trials on record: an open field | 1.00 |
| literature PubMed | 52 Parkinson's papers of 208 | 0.48 |
| pathways Reactome | 3 pathways; mostly energy | 0.60 |
Knock down and overexpress BST1 in a dopamine neuron, astrocyte and microglia tri-culture; measure oxygen consumption rate and ATP output and dopamine neuron survival after α-synuclein fibril challenge.
- Cell type
- a dopamine neuron, astrocyte and microglia tri-culture
- Direction
- Both arms: public data does not say which way helps.
- Molecular readout
- oxygen consumption rate and ATP output
- Functional readout
- dopamine neuron survival after α-synuclein fibril challenge
- Rule-out
- If neither arm moves oxygen consumption rate by 20% or more against non-targeting and empty-vector controls, across three independent differentiations, the hypothesis is wrong.
- Lab request
- CRISPRi knockdown and lentiviral overexpression of BST1 in a tri-culture of iPSC dopamine neurons, astrocytes and microglia; n = 3 differentiations; readouts: oxygen consumption rate and ATP output; dopamine neuron survival after α-synuclein fibril challenge. Controls: non-targeting guide, empty vector. About 8 to 10 weeks, most of it differentiation.
Earlier readings: 2026-10-26 0.66; 2026-10-25 0.66; 2026-10-24 0.66; 2026-10-23 0.66.
Sealed predictions
- BST1 in dopamine neurons, braak-0.14 (p 0.723) → +0.40 (p 0.288)receipt 0163
Swarm cases
receipt 0163: BST1 in dopamine neurons, braak
- power
- full sample -0.03 (p 0.922, n 18), 95% CI -0.49 to +0.45. the interval is wide: not enough donors to tell.
- confounder
- adjusted for sex: -0.03 (p 0.922, n 18). does not hold up.
- composition
- dopamine neurons share of nuclei vs axis +0.05 (p 0.854); controlling for it: -0.15 (p 0.555, n 18). could be the mix.
- skeptic
- leave-one-out range -0.18 to +0.09, sign flips 6; without severely affected donors -0.03 (p 0.922, n 18). fragile.
- cell type
- strongest in oligodendrocytes: -0.65 (p 0.0034, n 18) vs -0.03 in dopamine neurons. proposing oligodendrocytes.
- axis
- no measure beats "tracks Braak Lewy-body stage" for BST1 in dopamine neurons.
follow-up registered; not confirmed in substantia nigra, an independent cohort.
follow-up BST1 · oligodendrocytes · braak: substantia nigra, an independent cohort (Parkinson's or LBD vs controls) -0.40 p 0.155 (needs p < 0.0020)
Receipts
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